The effect of mesenchymal stromal cells of various origins on morphology of hippocampal CA1 area of rats with acute cerebral ischemia

Every year, about 150,000 strokes occur in Ukraine, and more than 100,000 people die from the consequences of stroke other circulatory disorders brain. So far, promising experimental data on treatment neurological dysfunction using mesenchymal stromal cells (MSCs) have been obtained. Purpose: to characterize impact MSCs various origins, lysate Wharton’s jelly-derived citicoline dynamics destructive changes hippocampal CA1 area rats with model acute cerebral ischemia according morphometric data. Materials methods. An experiment was performed 4-month-old male Wistar rats, which were subjected transient bilateral 20-minute ischemia-reperfusion (IR) internal carotid arteries. After modeling, animals injected intravenously MSCs, human rat adipose-derived at a dose 106 cells/animal. Other groups fetal fibroblasts cells/animal umbilical cord 0.2 mL/animal. Control mL saline. The last group received single reference drug 250 mg/kg. On 7th 14th day, total number neuron nuclei per 1 mm2 brain section counted area, ratio intact (karyorrhexis karyopyknosis) determined. Results. transplantation or contributed greater value that did not undergo pathological also increased. had most positive effect. this approached sham-operated animals. At same time, significantly exceeded signs destruction. Conclusion. A significant increase neurons without observed all during modeling ischemic injury after administration types studied cells, citicoline. result achieved MSCs.